Hi Mark,<br><br>Please try Philips REC format. This is a 4D raw format with integer. For both "raw" and "Philips REC", DtiStudio expect you to have 4D raw (no-header) file, not a bunch of 3D files. In your case, you need a single raw file that contains all 65 volume data (65 x 96 x 128 x 47) in one file. <br>
<br>For this conversion, you can read 4D Nifty or 4D analyze (depending on the version of DtiStudio) by MriView3D and save all the images at once as a "raw" format. Then, the images are ready for DtiMapping. In DtiMapping, as I mentioned, open it as Philips REC. In the opening window, specify;<br>
<br>96x128x47<br><br>Then you should get "65" as the number of the 3D images correctly calculated from the file size at the left bottom of the window. Also choose gradient-by-gradient for the order of the file. If you have a correct table with 65 lines, you should not get the error message.<br>
<br>If you have the 4D analyze file, the *.img file of the Analyze format can also be directly read as "Philips REC". However, you have to be careful because if the Analyze is in Neurology convention (default setup) and read the *.img as "Philips REC", the images may be inverted because DtiStudio uses Radiology convention.<br>
<br>I hope it helps.<br><br>Susumu<br><br><br><br><div class="gmail_quote">On Wed, Dec 16, 2009 at 7:39 PM, Mark A. Pinsk <span dir="ltr"><<a href="mailto:mpinsk@gmail.com" target="_blank">mpinsk@gmail.com</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
No luck. Let me outline my steps here:<br>
<br>
1. I start with a 4D NIFTI file that contains 65 volumes (60 DWI + 5 B0).<br>
The image dimensions are 96x128, 47 slices per volume.<br>
Slice orientation is Coronal. FOV is 96x128. Pixels are 1x1x1.1.<br>
<br>
2. I split the 4D NIFTI file into 65 3D NIFTI files using this FSL command:<br>
fslsplit input.nii.gz<br>
<br>
3. I convert each of the 65 3D NIFTI files to ANALYZE format:<br>
fslchfiletype ANALYZE vol0000.nii.gz<br>
[..]<br>
fslchfiletype ANALYZE vol0064.nii.gz<br>
<br>
4. I am able to successfully import the ANALYZE files in MRIView3D by<br>
choosing Analyze format (but no luck with Raw format).<br>
<br>
5. I have no luck importing the ANALYZE files in DTI Mapping choosing<br>
Raw format. I get this message:<br>
"Number of files in this fold is less than expected: (Img_Slices*Img_Blocks).<br>
<br>
Any suggestions? Thanks for all your help!<br>
Mark<br>
<br>
<br>
<br>
<br>
On Wed, Dec 16, 2009 at 1:34 PM, darshan pai <<a href="mailto:darshanp20@yahoo.com" target="_blank">darshanp20@yahoo.com</a>> wrote:<br>
> when you say a 4D file I assume that you have a Analyze file with an entry on the number of volumes , Is it? . If it is then try to make it a 3D file with all the 65 slices added in the z direction and see if it helps..<br>
><br>
> Regards<br>
><br>
> Dp<br>
><br>
><br>
><br>
> ----- Original Message ----<br>
> From: Mark A. Pinsk <<a href="mailto:mpinsk@gmail.com" target="_blank">mpinsk@gmail.com</a>><br>
> To: "DTI Studio, ROI Editor, Landmarker Questions/Support" <<a href="mailto:mristudio-users@mristudio.org" target="_blank">mristudio-users@mristudio.org</a>><br>
> Sent: Wed, December 16, 2009 12:13:42 AM<br>
> Subject: Re: [Mristudio-users] Using processed data<br>
><br>
> Also, I should mention that the Analyze file loads just fine using<br>
> "MRI View3D" and "Analyze" file format.<br>
><br>
><br>
> On Wed, Dec 16, 2009 at 12:09 AM, Mark A. Pinsk <<a href="mailto:mpinsk@gmail.com" target="_blank">mpinsk@gmail.com</a>> wrote:<br>
>> Thanks for your help, I'll do my best to answer them...<br>
>><br>
>> FYI: these are small volumes from animal scans ( 1 x 1 x 1.1 mm resolution):<br>
>><br>
>> Image Dimensions: 96 Width, 128 Height, 47 slices<br>
>> Slice Orientation: Axial<br>
>> Slice Sequencing: Inferior-Superior<br>
>> FOV: 96 Width, 128 Height, 1.1 Slice Thickness<br>
>> Pixel Size: 1.000 Width, 1.000 Height<br>
>> Slices to be processed: 0 to 46, All Slices<br>
>> Image Sequence: Gradient By Gradient<br>
>> b_Value: 1000<br>
>><br>
>> My gradient table has 65 entries. The first 5 entries are B0 images,<br>
>> the remaining 60 are for the 60 directions. There is no mean image at<br>
>> the end of my data. I can discard 4 of the B0 images, but that does<br>
>> not seem to make a difference on my error message. I have several B0<br>
>> images in accord with recent work that suggests having a ratio of 6:1<br>
>> diffusion to non-diffusion weighted images (Zhu et al. NeuroImage<br>
>> 2008).<br>
>><br>
>><br>
>><br>
>> On Tue, Dec 15, 2009 at 11:42 PM, Gonzalo Rojas <<a href="mailto:grojasy@puc.cl" target="_blank">grojasy@puc.cl</a>> wrote:<br>
>>> Hi Mark:<br>
>>><br>
>>> Many questions:<br>
>>><br>
>>> i) do you specify correctly the "image slices" quantity in "iamge dimension"<br>
>>> ?... that value is the quantitry of axial slices in each gradient<br>
>>> direction...<br>
>>><br>
>>> ii) and the "image width" and "image height" in "image dimension" ?... did<br>
>>> you checked that values ?...<br>
>>><br>
>>> ii) in "lice orientation" did you specify "axial" ?..<br>
>>><br>
>>> iii) and in "Voxel Size", "Field of View-width" and "Field of View-Heigth"<br>
>>> did you specifiy the FOV value correctly ?...<br>
>>><br>
>>> iv) you could check the previos values looking at "Pixel Size-Width" and<br>
>>> "Pixel Size-Height"...<br>
>>><br>
>>> v) in "Slices to be processed" it must appears 0-(image slices-1)<br>
>>><br>
>>> vi) in gradient table you must put the 61 (0-60) directions... in your 4D<br>
>>> analyze file is included the "mean" of all the 60 directions at the end of<br>
>>> the file ?... if it is, you must put "100,100,100" as the last gradient<br>
>>> direction to skip that image in the computation...<br>
>>><br>
>>> vii) in "Image secuence" you must select "Gradient by Gradient" option...<br>
>>><br>
>>> viii) in "Raw-BIN Files folder you must specify the IMG file of the Analyze<br>
>>> pair...<br>
>>><br>
>>> ix) why you have 5 b0 volumes ?... to compute tractography, you must have<br>
>>> only one b0 volume... you must put "100,100,100" in "Gradient Table" for<br>
>>> each of the 4 b0 slices to skipt them...<br>
>>><br>
>>> Sincerely,<br>
>>><br>
>>><br>
>>> Gonzalo Rojas Costa<br>
>>> Department of Radiology<br>
>>> Las Condes Clinic<br>
>>> Lo Fontecilla 441, Las Condes, Santiago, Chile.<br>
>>> Tel: 56-2-2105170<br>
>>> Cel: 56-9-97771785<br>
>>> <a href="http://www.clc.cl" target="_blank">www.clc.cl</a><br>
>>><br>
>>> El 16-12-2009 1:07, Mark A. Pinsk escribió:<br>
>>>><br>
>>>> Thanks !<br>
>>>><br>
>>>> I converted by 4D NIFTI file to a 4D ANALYZE file as you suggested.<br>
>>>><br>
>>>> I attempt to read it in as RAW, but it seems to get stuck...<br>
>>>><br>
>>>> I specify the file in the Raw-BIN Files Folder field, but I get this<br>
>>>> message:<br>
>>>> "Number of files in this fold is less than expected:<br>
>>>> (Img_Slices*Img_Blocks)."<br>
>>>><br>
>>>> I only have a single .img/.hdr pair in my folder since it is a 4D file<br>
>>>> (i.e. it contains 65 volumes (5 b0 volumes + 60 directions)).<br>
>>>><br>
>>>> Sorry, any help would be greatly appreciated.<br>
>>>> Thanks<br>
>>>> Mark<br>
>>>><br>
>>>><br>
>>>><br>
>>>><br>
>>>> On Tue, Dec 15, 2009 at 10:29 PM, Gonzalo Rojas<<a href="mailto:grojasy@puc.cl" target="_blank">grojasy@puc.cl</a>> wrote:<br>
>>>>><br>
>>>>> Hi Mark:<br>
>>>>><br>
>>>>> Yes... you could do all the processing that you mentioned (in FSL), and<br>
>>>>> bring it to dti-studio... the FSL output file format is NIFTI.GZ... you<br>
>>>>> must<br>
>>>>> convert it to ANALYZE 4D, and then you must load that ANALYZE 4D file as<br>
>>>>> a<br>
>>>>> RAW data file format in DTI-STUDIO...<br>
>>>>><br>
>>>>> Sincerely,<br>
>>>>><br>
>>>>><br>
>>>>> Gonzalo Rojas Costa<br>
>>>>> Department of Radiology<br>
>>>>> Las Condes Clinic<br>
>>>>> Lo Fontecilla 441, Las Condes, Santiago, Chile.<br>
>>>>> Tel: 56-2-2105170<br>
>>>>> Cel: 56-9-97771785<br>
>>>>> <a href="http://www.clc.cl" target="_blank">www.clc.cl</a><br>
>>>>><br>
>>>>> El 15-12-2009 23:37, Mark A. Pinsk escribió:<br>
>>>>>><br>
>>>>>> Hi, someone asked this a while ago in the archives but I didn't see a<br>
>>>>>> reply. Can I use data that I've processed in FSL (eddy current<br>
>>>>>> correction, motion correction, and field map-based undistortion) and<br>
>>>>>> bring it into dti-studio? The data is in one large 4D nifti file. I<br>
>>>>>> don't see where I can do this, is it not possible?<br>
>>>>>><br>
>>>>>> thanks<br>
>>>>>> mark<br>
>>>>>> _______________________________________________<br>
>>>>>> Mristudio-users mailing list<br>
>>>>>> <a href="mailto:Mristudio-users@mristudio.org" target="_blank">Mristudio-users@mristudio.org</a><br>
>>>>>> <a href="http://lists.mristudio.org/mailman/listinfo/" target="_blank">http://lists.mristudio.org/mailman/listinfo/</a><br>
>>>>>> Unsubscribe, send a blank email to:<br>
>>>>>> <a href="mailto:Mristudio-users-unsubscribe@mristudio.org" target="_blank">Mristudio-users-unsubscribe@mristudio.org</a><br>
>>>>>><br>
>>>>><br>
>>>><br>
>>>> _______________________________________________<br>
>>>> Mristudio-users mailing list<br>
>>>> <a href="mailto:Mristudio-users@mristudio.org" target="_blank">Mristudio-users@mristudio.org</a><br>
>>>> <a href="http://lists.mristudio.org/mailman/listinfo/" target="_blank">http://lists.mristudio.org/mailman/listinfo/</a><br>
>>>> Unsubscribe, send a blank email to:<br>
>>>> <a href="mailto:Mristudio-users-unsubscribe@mristudio.org" target="_blank">Mristudio-users-unsubscribe@mristudio.org</a><br>
>>>><br>
>>><br>
>><br>
><br>
> _______________________________________________<br>
> Mristudio-users mailing list<br>
> <a href="mailto:Mristudio-users@mristudio.org" target="_blank">Mristudio-users@mristudio.org</a><br>
> <a href="http://lists.mristudio.org/mailman/listinfo/" target="_blank">http://lists.mristudio.org/mailman/listinfo/</a><br>
> Unsubscribe, send a blank email to: <a href="mailto:Mristudio-users-unsubscribe@mristudio.org" target="_blank">Mristudio-users-unsubscribe@mristudio.org</a><br>
><br>
><br>
><br>
><br>
><br>
> _______________________________________________<br>
> Mristudio-users mailing list<br>
> <a href="mailto:Mristudio-users@mristudio.org" target="_blank">Mristudio-users@mristudio.org</a><br>
> <a href="http://lists.mristudio.org/mailman/listinfo/" target="_blank">http://lists.mristudio.org/mailman/listinfo/</a><br>
> Unsubscribe, send a blank email to: <a href="mailto:Mristudio-users-unsubscribe@mristudio.org" target="_blank">Mristudio-users-unsubscribe@mristudio.org</a><br>
><br>
<br>
_______________________________________________<br>
Mristudio-users mailing list<br>
<a href="mailto:Mristudio-users@mristudio.org" target="_blank">Mristudio-users@mristudio.org</a><br>
<a href="http://lists.mristudio.org/mailman/listinfo/" target="_blank">http://lists.mristudio.org/mailman/listinfo/</a><br>
Unsubscribe, send a blank email to: <a href="mailto:Mristudio-users-unsubscribe@mristudio.org" target="_blank">Mristudio-users-unsubscribe@mristudio.org</a><br>
<br>
</blockquote></div><br>