Hi everyone,<br><br>I'm in the process of reorienting subjects to a more standard space using 7 landmarks across subjects. For the most part the transformation matrix saved post rigid/affine transformation is reorienting fiber tracts perfectly (we're confirming this using FSL). However some subject's fiber tracts are going way off when we apply their respective transformation matrices. They'll be slanted in the axial view, etc. Something I've noticed is that the subjects whose tracts are mis-rotated are loading into landmarker upside down and require me to remove a (-) sign from their raw image information. However, not all subjects that load upside down are resulting in fiber tracts that are mis-oriented.<br>
<br>Any suggestions as to why this might be occurring?<br><br>Thanks,<br><br>Paul<br>